FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n01_B.F1.mhWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n01_B.F1.mhWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32272138
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT635720.19698725879270843TruSeq Adapter, Index 27 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG250900.021.16245348
TGCTTGA242350.020.57927560
GTATGCC265350.020.12873647
GCTTGAA256250.020.1186361
CTGCTTG254300.019.51575759
CGTATGC273150.019.45134446
ATGCCGT275500.019.17114449
TCGTATG291200.018.16146345
TGCCGTC309950.017.0742650
GCCGTCT311550.016.54840351
CTCGTAT319100.016.05700144
CCGTCTT363500.014.41363752
TCTCGTA364500.013.60568543
TCTGCTT375200.013.56358
CTTGAAA412700.012.83104362
TATCTCG394500.012.63323141
ATCTCGT432350.011.52718542
CTTCTGC465300.011.23006456
TTCTGCT461350.011.15169757
TTATCTC479050.010.44023540
Produced by FastQC (version 0.11.5)