FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n01_B.F1.mbWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n01_B.F1.mbWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31884932
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT569200.17851692454605203TruSeq Adapter, Index 21 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG139550.033.63441548
GTATGCC144100.033.42253547
CGTATGC143900.032.13146
TCGTATG150100.028.49504345
ATGCCGT152700.027.39132949
CTCGTAT178550.024.81548144
TGCCGTC185550.020.82535450
TCTCGTA218850.020.3737243
AATCTCG306500.019.13781541
CGCGTAT60750.018.89628444
GCGTATG58100.018.79564745
AATCGCG123800.018.57349241
TCGCGTA64700.017.74259243
CGGAATC547950.017.50115438
ATCTCGT287300.017.49313442
GGAATCT388050.017.01023539
GCCGTCT188900.016.87990651
ATCGCGT89400.016.87285642
TGCTTGA184300.016.25566760
GCGTGAA111050.015.695209561
Produced by FastQC (version 0.11.5)