FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n01_B.F1.ffWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n01_B.F1.ffWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39836685
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1479450.37137879319024664TruSeq Adapter, Index 25 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA400500.028.4537861
TGCTTGA410200.027.55909760
CTGCTTG467500.024.2335859
GTATGCC513100.022.27916747
TATGCCG515800.022.17617248
CGTATGC537100.021.38779846
CTTGAAA539000.021.33709362
ATGCCGT542600.021.10015149
TCGTATG548050.020.9475445
GCCGTCT554650.020.40191751
TGCCGTC570600.020.03407150
CTCGTAT566750.019.67454744
TCTGCTT598000.019.05044758
CCGTCTT602150.018.91922452
TCTCGTA630400.017.54366343
TTCTGCT672500.016.8827857
CTTCTGC712950.016.16048856
TATCTCG696750.015.762567541
ATCTCGT735950.015.01805642
GTCTTCT804100.014.35026454
Produced by FastQC (version 0.11.5)