FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n01_B.F1.fdWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n01_B.F1.fdWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43900450
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT1740160.3963877363443883TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG424400.031.94182448
GTATGCC432000.031.38798347
CGTATGC453950.029.91615746
ATGCCGT455150.029.7837649
TGCTTGA445400.029.49037760
CTGCTTG452550.028.92374459
GCTTGAA465350.028.51947861
TCGTATG476900.028.38810245
TGCCGTC505650.026.8023250
CTCGTAT503000.026.02964244
GCCGTCT525950.025.44168951
TCTCGTA561050.022.86846243
CCGTCTT588450.022.71430652
TCTGCTT593550.022.13535358
TATCTCG592400.021.52842741
CTTGAAA673200.020.10395662
ATCTCGT648500.019.79547342
ATATCTC674600.018.9208640
CTTCTGC710450.018.77391856
TTCTGCT703750.018.68410557
Produced by FastQC (version 0.11.5)