FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n01_B.F1.fcWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n01_B.F1.fcWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41484493
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT605720.14601118543259042TruSeq Adapter, Index 22 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG265450.020.14743648
GTATGCC277350.019.48490547
CGTATGC295550.018.48625846
ATGCCGT296700.017.95453549
CTGCTTG287400.017.6089759
TCGTATG309850.017.38445345
GCTTGAA304550.017.2150161
TGCTTGA299700.017.20160760
TGCCGTC338100.015.88026650
GCCGTCT338950.015.45835451
CTCGTAT341350.014.92838844
TCTCGTA396850.012.69066243
CCGTCTT415450.012.66188852
TCTGCTT428950.012.11634858
ATCTCGT466750.010.94757442
TAATCTC460550.010.89763340
AATCTCG492550.010.26763741
GTAATCT492200.010.18983139
CTTGAAA541550.09.99135662
CTTCTGC533950.09.9631156
Produced by FastQC (version 0.11.5)