FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n01_B.F1.faWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n01_B.F1.faWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30189150
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT2793650.9253821323223742TruSeq Adapter, Index 20 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG465550.049.3786245
GTATGCC483450.048.7599547
CGTATGC485650.048.54586846
CTCGTAT460750.048.0904744
GCTTGAA477350.046.71910561
TGCTTGA468150.046.45660
CTGCTTG468900.046.0232759
TATGCCG516700.045.6764848
TCTCGTA475450.044.62337543
GCCGTCT502700.044.58816551
ATGCCGT529350.044.16169449
CCGTCTT525350.042.77047352
TGCCGTC548550.042.30963550
ATCTCGT511350.041.48375342
TATCTCG521100.040.7549341
TCTGCTT541150.040.0661158
CTTGAAA562400.040.00234662
GTCTTCT607800.035.90263754
CTTCTGC606200.035.85907456
TTCTGCT599800.035.3666157
Produced by FastQC (version 0.11.5)