FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n01_A.F1.mfWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n01_A.F1.mfWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35978830
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTAT882850.24538040842350906TruSeq Adapter, Index 16 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG296900.028.35266348
GTATGCC305450.027.75371647
CGTATGC306100.027.14572746
ATGCCGT328200.025.0940749
TCGTATG310350.024.68738645
GCTTGAA302350.023.77617561
TGCTTGA299250.022.81778160
CTCGTAT334800.022.38094944
TGCCGTC355700.022.219250
CTGCTTG307700.021.98639759
GCCGTCT354000.020.68458251
TCTCGTA369150.019.2269343
CCGTCTT407400.018.19551352
CGCATCT437950.016.97408539
ATCTCGT436200.016.41597442
CCGCATC514400.016.18649738
TCTGCTT429600.016.14685258
GCATCTC461850.015.8834340
CTTGAAA482350.015.3823762
CATCTCG488650.014.96193741
Produced by FastQC (version 0.11.5)