FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n01_A.F1.meWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n01_A.F1.meWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38736984
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT769060.1985337836316839TruSeq Adapter, Index 15 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG235600.025.58229848
GTATGCC247650.025.04419547
CGTATGC250000.024.45864146
TCGTATG262050.022.42555245
ATGCCGT258100.021.9824549
CTCGTAT302450.020.1924944
TGCCGTC285600.018.38290650
TGCTTGA249100.017.97014460
GCTTGAA279400.017.96294461
TCTCGTA357500.017.05373843
CTGCTTG249100.016.2137359
GCCGTCT284100.015.99130951
AATCTCG492500.015.3069341
ATCTCGT459700.014.78501142
CCGTCTT361050.013.33851552
GCGTGAA134200.013.0659261
CGCGTAT81900.012.81992244
GCGTATG75000.012.69358445
GAATCTC618200.012.51737940
TGCGTGA121700.011.67589760
Produced by FastQC (version 0.11.5)