FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n01_A.F1.fhWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n01_A.F1.fhWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31846640
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT840480.2639148117352411TruSeq Adapter, Index 19 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG255550.030.92601648
GTATGCC255550.030.85753447
CGTATGC264100.029.95122746
TCGTATG275850.028.53577245
ATGCCGT287550.027.53310249
CTCGTAT275400.026.36976244
TGCTTGA294700.026.08008660
CTGCTTG295400.025.9115759
GCTTGAA314400.024.80223561
GCCGTCT325700.024.1468951
TGCCGTC329700.023.92828450
TCTCGTA303250.022.57456643
CCGTCTT374600.021.13403152
ACGATCT338300.019.41877439
TCTGCTT407100.019.14584758
ATCTCGT347000.019.13327242
GATCTCG359750.018.53313841
CGATCTC357650.018.45617340
AACGATC375550.017.71639338
CTTGAAA446700.017.67583562
Produced by FastQC (version 0.11.5)