FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n01_A.F1.fgWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n01_A.F1.fgWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32444478
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT992110.3057870125079528TruSeq Adapter, Index 18 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC300650.029.26767547
TATGCCG302150.029.13400548
CGTATGC303550.029.12629746
TCGTATG312350.027.87967145
TGCTTGA303550.027.63684560
ATGCCGT321050.027.3316949
CTGCTTG311650.026.96338359
GCTTGAA319800.026.91105761
CTCGTAT320400.025.87735644
TGCCGTC359250.024.33775350
GCCGTCT357100.024.1313951
TCTCGTA352150.022.73915543
CCGTCTT406200.021.34231252
TCTGCTT418400.020.4269458
ATCTCGT397650.019.53003342
CTTGAAA478200.018.443462
CGCACAT449150.018.35906837
GCACATC440800.017.95236638
CACATCT444950.017.5725239
CATCTCG446700.017.50316441
Produced by FastQC (version 0.11.5)