FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n01_A.F1.fdWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n01_A.F1.fdWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31798385
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT2797340.8797113438308267TruSeq Adapter, Index 14 (97% over 44bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC482850.048.16167447
TATGCCG481350.048.0936948
CGTATGC488400.047.449446
ATGCCGT493850.045.77073349
TCGTATG484250.045.7090645
CTCGTAT496900.043.39405444
TGCCGTC525150.042.2696350
TGCTTGA471450.042.2257560
GCTTGAA501850.041.4532961
CTGCTTG475650.041.11702359
GCCGTCT514850.040.58633451
TCTCGTA526550.039.66100743
TATCTCG541400.039.20044341
CGTATCT556200.038.82551239
GTATCTC547900.038.80663340
CCGTCTT572500.036.79043252
CCGTATC633950.036.53727338
ATCTCGT588250.035.78664442
TCCGTAT686400.035.15295437
TCTGCTT589650.033.54149258
Produced by FastQC (version 0.11.5)