FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n01_A.F1.fcWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n01_A.F1.fcWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34769333
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT844800.24297273692308105TruSeq Adapter, Index 13 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA282050.025.51250661
TGCTTGA291250.024.35812860
CTGCTTG309450.022.69916359
GTATGCC344600.021.06584747
CGTATGC350150.020.78171746
TCGTATG352950.020.34895545
TATGCCG369950.019.6507448
CTCGTAT342950.019.62448744
GCCGTCT383900.018.66313451
ATGCCGT388700.018.63977449
CTTGAAA391350.018.61059862
TGCCGTC410450.017.68612750
TCTCGTA378550.017.46454643
CCGTCTT421050.017.09028252
TCTGCTT430000.016.56330758
ATCTCGT430700.015.20357242
TTCTGCT495000.014.39541957
AATCTCG460700.014.28199441
CTTCTGC508550.014.26651856
GTCTTCT569300.012.78707954
Produced by FastQC (version 0.11.5)