FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25487569
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13131125.151970358569701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG62200.017.3716641
GAGGGGG60600.017.1678312
ATGCCGT117350.015.00052746
AGGGGGG75250.013.8595823
CAGTCAC270600.013.08805724
CTCGTAT126800.012.72340341
TATGCCG145800.012.52952545
CTCCAGT309900.011.99293321
GCCGTCT128900.011.97311948
CCAGTCA299400.011.86412923
TGCCGTC147550.011.47962747
TCCAGTC320500.011.33420222
ACTCCAG336250.011.24054420
TCTCGTA147550.010.79179840
GAACTCC357200.010.53299518
GTATGCC184400.010.49508244
GTCACGA90600.010.23623326
CTGAACT378950.010.1872316
ATCGGGG27300.010.12712339
CGTCTGA381600.010.09810313
Produced by FastQC (version 0.11.5)