FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ps-bZIP9..ph-bZIP9_Col..h-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ps-bZIP9..ph-bZIP9_Col..h-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4810847
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT103140.21439052208478052TruSeq Adapter, Index 19 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCC94930.19732492012321323TruSeq Adapter, Index 19 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32100.030.20217545
ATGCCGT34000.028.41146746
CGTATGC34850.028.3210843
TGCCGTC33400.028.18832847
GCCGTCT31600.028.02185648
GTATGCC35150.027.78064544
TCGTATG38950.024.44133442
CCGTCTT38050.023.54771649
CTCGTAT38150.023.4859941
GCTATCT38700.021.97651336
TCTCGTA40400.021.48492240
TATCTCG40850.020.73417538
AGCTATC43300.020.69262335
CTGCTTG39300.020.12701456
AAGGGGG10250.019.8046770
CGTCTTC45100.019.47873150
ATCTCGT46350.018.42483139
CTTCTGC44650.018.10731153
CCTGAAG58350.017.45482830
CTGAAGC59100.017.2333231
Produced by FastQC (version 0.11.5)