FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ps-bZIP9..ph-bZIP63_Col..h-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ps-bZIP9..ph-bZIP63_Col..h-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15546837
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33150.017.42065845
ATGCCGT36400.014.99986646
CGTATGC42250.014.57975143
GTATGCC41200.014.10181544
GCCGTCT39350.013.43062948
TGCCGTC42100.013.0521447
CTCGTAT50600.011.4821141
TCGTATG54900.011.220342
GCTATCT57800.08.71964536
CCGTCTT62850.08.68727249
TCTCGTA65900.08.55075740
TATCTCG63600.08.30967338
CTGCTTG73200.07.31550856
AGCTATC72200.07.271403335
CCTGAAG90800.06.976809530
CTATCTC85050.06.66660737
CTGAAGC93350.06.59876231
CACCTGA93850.06.4890228
ATCTCGT88200.06.388831639
GCTTGAA93550.06.24793958