FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ps-bZIP9..ph-bZIP25_Col..h-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ps-bZIP9..ph-bZIP25_Col..h-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10851400
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT401110.3696389405975266TruSeq Adapter, Index 19 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCC375420.3459645759994102TruSeq Adapter, Index 19 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG118700.029.13194345
GCCGTCT112100.028.63037351
TGCCGTC118650.028.52475747
ATGCCGT119950.028.44903846
GTATGCC122250.028.25735547
CGTATGC122850.028.23330746
CTCGTAT121550.026.34689141
TCGTATG131150.026.12628445
CCGTCTT128250.025.18880752
TCTCGTA127400.023.98325240
CTGCTTG122750.023.8082959
TATCTCG130200.023.52124438
GCTATCT131150.023.45761336
AGCTATC142000.022.50327335
ATCTCGT136100.022.50158739
AAGGGGG26100.022.26029270
CGTCTTC143300.021.81064650
CTTCTGC143900.020.8684356
CTATCTC156950.019.84686537
GCTTGAA154950.019.69645961
Produced by FastQC (version 0.11.5)