FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ps-bZIP9..ph-bZIP10_Col..h-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ps-bZIP9..ph-bZIP10_Col..h-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12875378
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25800.018.31372845
ATGCCGT29150.016.3291346
GTATGCC31700.015.78844844
CGTATGC32100.015.04654443
GCCGTCT30100.014.30214948
TGCCGTC34100.013.85613447
TCGTATG41550.011.45593642
CTCGTAT38700.011.3047741
CCGTCTT49700.09.50692649
GCTATCT47950.09.488934536
AGCTATC58100.08.79507335
TATCTCG53950.08.10926138
CCTGAAG68300.07.994107230
TCTCGTA56950.07.98936640
CTGAAGC74500.07.798591631
TCACCTG71100.07.72848627
CACCTGA65350.07.712282728
GTCACCT68850.07.676042626
CTGCTTG57900.07.556038456
CTATCTC70900.06.713598337