FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ps-bZIP63..ph-bZIP9_Col..h-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ps-bZIP63..ph-bZIP9_Col..h-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13349026
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT193770.1451566578715181TruSeq Adapter, Index 19 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCC190470.14268456739840046TruSeq Adapter, Index 19 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG68450.025.974948
ATGCCGT69500.024.87744349
GCCGTCT69800.024.11866651
GTATGCC74700.024.035947
CGTATGC75300.023.84437846
TGCCGTC74550.023.33309650
TCGTATG84300.021.00809145
CTCGTAT81100.020.49909844
CCGTCTT93050.018.01694552
GCTATCT92900.017.06650239
TATCTCG94150.016.76556641
TCTCGTA94050.016.70896543
CTGCTTG95050.016.68046259
AGCTATC104600.015.49213638
CTTCTGC113150.014.5070956
GCTTGAA114950.014.37126761
CGTCTTC120000.014.08730353
CTATCTC115100.014.01804940
ATCTCGT115100.013.74437842
TGCTTGA122700.013.52059460
Produced by FastQC (version 0.11.5)