FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ps-bZIP53..ph-bZIP10_Col..s-B..h-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ps-bZIP53..ph-bZIP10_Col..s-B..h-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5271741
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCC54220.10285027280361458TruSeq Adapter, Index 7 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19700.027.18238845
GCCGTCT18400.026.63008948
ATGCCGT19850.026.09537746
GTATGCC20600.025.99480844
TGCCGTC19450.025.91225847
CGTATGC20750.025.4695543
CCGGCTA22700.024.20673630
ACCGGCT22800.023.7935529
CACCGGC22700.023.74418628
CGGCTAT22950.023.63803531
GTCACCG24550.021.95490626
GGCTATG25650.021.42272232
TCGTATG25750.021.33952742
CTCGTAT24650.020.02002341
AGTCACC29100.018.64236825
CCGTCTT26750.018.57919749
GATCTCG23750.018.42081338
TCACCGG29850.018.05671727
CAGTCAC29750.017.99976524
CTGCTTG25600.017.6364956
Produced by FastQC (version 0.11.5)