FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ps-bZIP44..ph-vec_Col..h-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ps-bZIP44..ph-vec_Col..h-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5212010
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAT51650.025.81762337
GCGCATC47450.025.74250635
CATCGCG15450.025.1452641
ATGCGCA57950.025.12473536
TATGCCG16650.024.38402245
AATGCGC61850.024.10635435
GTATGCC19100.022.72217844
CGCATCG28250.022.0527739
TAATGCG67300.022.05040634
GCATCGC18300.021.99421140
TGCCGTC14200.021.93629650
CGTATGC16200.021.82066543
CGCGTAT9750.021.53814144
ATCGCGT12600.021.11079842
GCCGTCT11800.021.0590151
ATGCCGT15800.020.6009646
TCGCGTA10600.020.47139543
ATGCCGG8550.019.23948146
CGCATCT33400.018.96678536
CTAATGC80000.018.76865233
Produced by FastQC (version 0.11.5)