FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ps-bZIP25..ph-bZIP9_Col..h-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ps-bZIP25..ph-bZIP9_Col..h-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863958
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT62700.21892779153884237TruSeq Adapter, Index 19 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCC58810.2053451901180115TruSeq Adapter, Index 19 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19750.028.70845448
GCCGTCT19450.028.0715851
ATGCCGT20400.027.79372249
TGCCGTC20450.027.72576950
GTATGCC21000.027.66627347
CGTATGC20500.027.65814246
CTCGTAT21200.024.76379844
TCGTATG23450.024.1787645
CCGTCTT23250.023.93514352
CTGCTTG22350.022.55001459
GCGCCCG952.7830782E-422.10494831
TATCTCG23850.020.98502741
GCTATCT24550.020.67180439
TCTCGTA24900.020.24067540
CGTCTTC27400.020.18219453
AGCTATC25500.020.17618438
CTTCTGC27250.019.26578156
CCTGAAG31800.019.26073333
CTGAAGC32350.018.608734
CACCTGA32150.018.50673731
Produced by FastQC (version 0.11.5)