FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ps-bZIP2..ph-vec_Col..h-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ps-bZIP2..ph-vec_Col..h-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5951740
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC41150.028.40786438
TGCGCAT45200.027.25625437
ATGCGCA49050.026.4012636
AATGCGC51500.026.09672235
TGCCGTC13050.024.13759250
ATGCCGT13850.023.75417949
TAATGCG57600.023.69758234
TATGCCG15300.023.5617648
CATCGCG13900.023.16514241
GTATGCC17550.022.13644247
CGTATGC14900.022.08022746
CGCATCG27500.021.5087939
GCATCGG13000.020.19202437
ATCGCGT11700.019.74331342
TCGCGTA9400.019.73376743
CGCGTAT10200.019.55854844
GCCGTCT11600.019.31007451
CGCATCT31400.019.06024239
CTAATGC71900.019.03311233
CATCGGG13000.018.8458938