FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ps-bZIP11..ph-vec_Col..h-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ps-bZIP11..ph-vec_Col..h-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5835568
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC51750.027.12035635
TGCGCAT57100.026.47945234
ATGCGCA64200.025.94975733
AATGCGC68000.025.42607932
CGCATCG30200.024.22148136
TAATGCG72900.023.71705631
CATCGCG17850.023.33297338
CATCGGG12400.022.8625538
GTATGCC20700.022.82573347
TATGCCG19400.022.1903848
CGTATGC18950.021.7938646
ATGCCGT17000.021.61731349
GCATCGG14000.020.99967437
TGCCGTC16850.020.97890750
CGCATCT38050.020.9720839
ATCGGGG7950.020.25125939
TGCCGGC9700.020.20587347
GCATCGC21600.020.09228337
ATCGCGG9950.020.04994239
TCGCGTA10700.019.95296343