FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ps-bZIP10..ph-bZIP9_Col..h-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ps-bZIP10..ph-bZIP9_Col..h-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5654745
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT117120.20711809285829866TruSeq Adapter, Index 19 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCC107060.1893277238849851TruSeq Adapter, Index 19 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG39250.029.51565748
GCCGTCT37600.029.04229451
TGCCGTC40050.028.13957250
ATGCCGT40900.027.98263449
CGTATGC42950.027.21745146
GTATGCC42650.027.08064847
CTCGTAT43450.025.45431944
TCGTATG47100.024.74500345
CCGTCTT45150.024.18583152
GCTATCT47150.021.97223339
TCTCGTA47400.021.48714843
TATCTCG47900.021.11672241
CTGCTTG46000.020.99981156
CGTCTTC55350.020.04498753
ATCTCGT52950.019.43325442
AGCTATC53900.019.28554238
CTTCTGC55400.018.57384156
CCTGAAG68350.017.76867733
CACCTGA68350.017.61505731
CTGAAGC70200.017.35027134
Produced by FastQC (version 0.11.5)