FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ps-bZIP1..ph-vec_Col..h-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ps-bZIP1..ph-vec_Col..h-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6681898
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC43250.025.24837935
TGCGCAT47750.025.14118434
ATGCGCA53000.024.36775433
AATGCGC56350.023.7886532
TATGCCG16600.021.71671548
TAATGCG63700.021.42842131
CGCATCG26700.021.1047239
CATCGCG15450.021.06781441
GTATGCC18550.020.18853847
TGCCGTC14650.020.06811750
ATGCCGT16000.019.6873649
CGTATGC17050.019.29605146
GCCGTCT13100.019.23650651
ATCGCGT12500.019.03986742
GCATCGC18600.018.25255840
CGCGTAT11450.018.03480744
CTAATGC77900.017.74698630
ATGCCGG8850.017.40100746
CGCATCT35100.017.2505936
TCGCGTA11950.016.10867143