FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ps-bZIP1..ph-bZIP11_Col..s-B..h-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ps-bZIP1..ph-bZIP11_Col..s-B..h-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9183684
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCG48550.027.97098730
TCCGCGA49700.027.32377231
CCGCGAA50300.026.58034932
CGCGAAA50100.025.91833
CACTCCG55700.024.44329328
ACTCCGC56600.024.17829329
GCGAAAT56850.022.77910234
GTCACTC67750.020.76739726
CAGTCAC68350.020.58509424
CCAGTCA71050.019.7043123
TCACTCC72950.019.04717327
AGTCACT77900.018.42093725
TCCAGTC76150.018.38465122
ACTCCAG76200.018.32675620
TATGCCG15000.017.96655345
CTCCAGT80200.017.76173421
ATCGGGG9750.016.87168939
ATGCCGT14500.016.65506746
ACGTCTG88650.015.91165212
GAACTCC86800.015.88768818