FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP9..ps-bZIP53_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP9..ps-bZIP53_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6201932
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT147240.2374098909823584TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC121890.19653553118608846TruSeq Adapter, Index 7 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG43350.029.38824348
GTATGCC46200.027.87835747
ATGCCGT42600.027.85168349
CGTATGC44900.027.67217846
GCCGTCT34400.027.47050751
TGCCGTC41150.027.38718450
CTCGTAT47950.025.10930444
CGTATCT59350.023.29384639
TCGTATG50350.023.2868245
TCGTATC71900.022.5867638
TATCTCG55900.022.35225141
TCTCGTA52050.022.32452443
CCGTCTT45050.022.29710452
TATCGCG11250.020.8442938
GTATCTC61950.020.79080840
ATCTCGT59750.020.50194242
ATTCGTA90700.020.45188936
TTCGTAT87500.020.27985237
GAATTCG99350.019.19981234
CACGAAT100800.018.85402931
Produced by FastQC (version 0.11.5)