FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP9..ps-bZIP53_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP9..ps-bZIP53_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6849975
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT165570.24170891134639177TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC132730.19376713053697275TruSeq Adapter, Index 7 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG46850.030.92824248
ATGCCGT46200.029.77248249
GCCGTCT39000.029.70488451
CGTATGC47450.029.65202146
GTATGCC50050.028.8109547
TGCCGTC44750.028.78188550
CTCGTAT53400.025.16833344
TCGTATC74850.024.87621338
CCGTCTT51200.024.54081352
CGTATCT64150.024.06059639
TCGTATG55750.023.60518645
TATCTCG61350.023.5042941
TCTCGTA60000.021.69982143
ATCTCGT65150.021.48869542
GTATCTC68800.021.16261740
ATTCGTA97050.021.13326336
TTCGTAT95150.020.7460237
TATCGCG12850.019.88310441
CTGCTTG45950.019.8801459
TGCCGGC7050.019.85799250
Produced by FastQC (version 0.11.5)