FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP9..ps-bZIP44_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP9..ps-bZIP44_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15781783
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT163840.10381589963567488TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG63450.024.98792848
ATGCCGT68050.022.5787649
GTATGCC71950.022.0845647
CGTATGC71700.021.38053946
GCCGTCT61350.020.87998651
TGCCGTC71500.020.02077950
TCGTATG80100.018.133445
CTCGTAT74050.017.53527344
CCGTCTT85600.015.53722852
CATTCAG144100.015.42316233
TCTCGTA94700.013.85941743
CACATTC180250.012.38822531
CTGCTTG87150.012.20871359
ATTCAGA187550.011.88737834
CAGAAAT183650.011.81583537
TTCAGAA188700.011.75928835
CTCCAGT187750.011.72558124
ACTCCAG190050.011.60209423
GCTTGAA108200.011.51559561
ACATTCA196300.011.42882132
Produced by FastQC (version 0.11.5)