FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP9..ps-bZIP44_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP9..ps-bZIP44_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10001370
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41100.024.78068748
ATGCCGT43050.021.46312549
GTATGCC46500.021.22552147
CGTATGC45700.020.90781446
GCCGTCT38800.019.75488951
TGCCGTC45650.019.47399950
TCGTATG47300.018.79467245
CTCGTAT46750.017.06928844
CATTCAG84050.016.1151933
CCGTCTT49450.015.78340652
TCTCGTA58700.013.35586643
CACATTC111600.012.51327231
CTCCAGT114050.011.90695824
ATTCAGA118050.011.74062934
CTGCTTG54350.011.59137959
ACATTCA122000.011.56131932
TTCAGAA119400.011.54925835
ACTCCAG117750.011.53275323
CAGTCAC120100.011.36543327
CAGAAAT114700.011.16811537