FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP9..ps-bZIP2_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP9..ps-bZIP2_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814890
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT65170.23151881601057236TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC57600.20462611327618485TruSeq Adapter, Index 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18900.029.07377848
TGCCGTC18000.028.58304247
CGTATGC19950.027.71901946
ATGCCGT19350.027.49326149
GCCGTCT18350.026.70272648
GTATGCC20600.026.50458547
TCGTATG23200.023.98682645
CTCGTAT21050.023.9427544
CGCTCAT25100.023.42647434
CCGTCTT22550.022.19489752
TATCTCG23550.021.2524438
CCGCTCA27350.021.24330733
ACCGCTC27650.021.0128232
TCTCGTA23400.020.79038843
CTGCTTG23800.020.58802659
CACCGCT28800.020.29529431
GCTCATT31200.019.29467635
ATCTCGT25450.019.25324239
TCACCGC30100.019.18619230
CGTCTTC26100.018.77375650
Produced by FastQC (version 0.11.5)