FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP9..ps-bZIP2_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP9..ps-bZIP2_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3668252
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT106990.2916648038357234TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC100950.27519919569320755TruSeq Adapter, Index 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC30100.030.81351546
GTATGCC30800.030.11320947
GCCGTCT28250.030.10576451
TATGCCG30350.030.0984248
TGCCGTC29600.029.91511550
ATGCCGT29700.029.57870349
CTCGTAT31550.028.39896244
TCGTATG34650.026.26225345
CCGTCTT32550.026.23618752
TATCTCG35250.025.21949841
TCTCGTA35050.024.86412843
CGCTCAT39900.024.64877134
CCGCTCA41700.023.83659433
CTGCTTG35350.023.6630359
ATCTCGT38600.022.75874542
CGTCTTC37750.022.34405353
ACCGCTC43850.022.26878432
CTTCTGC39850.021.34223256
GCTCATT48350.020.99246435
CACCGCT46200.020.83303831
Produced by FastQC (version 0.11.5)