FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP9..ps-bZIP1_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP9..ps-bZIP1_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4275415
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG28200.030.40755842
TCGGGGG23350.028.32953643
CGGGGGT11350.027.44471444
AATCGGG34750.026.08612341
GAATCGG37750.024.29119740
ACTCCGG52550.023.4441532
TCCGGAG52600.023.35532834
CACTCCG53900.022.92189631
CTCCGGA54400.022.64687733
CCGGAGA54700.022.13876335
CGGAGAA55800.021.26327136
AGAATCG46100.020.95427939
TCACTCC62900.019.75341830
GTCACTC62700.019.42568229
CGGGGGG19050.019.10745844
GGAGAAT60500.018.85935437
CAGTCAC66000.018.24227727
AGTCACT68200.017.96173328
TCGGGGT8800.017.4998643
CCAGTCA68850.017.3854826