FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP9..ps-bZIP11_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP9..ps-bZIP11_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17530466
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG50500.020.51462248
ATGCCGT53550.019.08475549
GCCGTCT51950.018.4598551
CGTATGC60050.017.7183746
TGCCGTC58400.017.32001150
GTATGCC60400.016.92034147
CTCGTAT64050.015.46439644
TCGTATG70300.014.935863545
CCGTCTT82000.011.90840252
TCTCGTA80800.011.21899843
CACGAGA103150.010.62033731
CTGCTTG85550.010.064177559
TCACGAG107950.010.018414530
GTCACGA114250.09.61914829
AGTCACG118800.09.25073928
CGAGATT120400.09.18596933
ACGAGAT132250.08.52166832
CGTCTTC117250.08.41781653
AGATTCC137250.08.26222635
GCTTGAA111350.08.17233861
Produced by FastQC (version 0.11.5)