FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP9..ps-bZIP11_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP9..ps-bZIP11_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13478006
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35750.019.18862345
ATGCCGT38450.017.93220546
GTATGCC42100.016.8763244
CGTATGC42750.016.3741143
TGCCGTC43250.016.02296447
GCCGTCT40900.015.91671648
TCGTATG50650.014.16570742
CTCGTAT48400.013.305655541
CCGTCTT61600.011.2498949
TCACGAG80450.010.13666927
CACGAGA76800.010.02598128
GTCACGA85250.09.44275926
TCTCGTA63150.09.36649640
AGTCACG85000.09.34700325
CTGCTTG65950.09.28724956
CGAGATT92200.08.35135930
CTTCTGC82500.08.14537553
ACTCCAG102500.08.05863720
CGTCTTC87800.07.852998350
GCTTGAA83300.07.8150558
Produced by FastQC (version 0.11.5)