FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP63..ps-bZIP53_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP63..ps-bZIP53_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5269966
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT120120.22793315934106595TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC92610.17573168403743022TruSeq Adapter, Index 7 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32950.030.69769948
ATGCCGT32500.028.53815549
GCCGTCT28600.028.39130451
CGTATGC34550.028.26308446
TGCCGTC31350.027.91038950
GTATGCC37050.027.8674547
CTCGTAT36850.024.78942344
TCGTATG38450.023.57581945
TCGTATC53200.023.55238238
CGTATCT45750.022.87407339
CCGTCTT37500.022.49309352
ATTCGTA65350.021.42287636
TATCTCG46250.021.41598741
TCTCGTA42300.021.2645543
CACGAAT69350.020.89379331
TTCGTAT65250.020.6511237
GAATTCG69550.020.58209434
GTATCTC49850.020.29065340
GTCACGA71650.020.17424429
AGTCACG73650.019.72144528
Produced by FastQC (version 0.11.5)