FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP63..ps-bZIP53_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP63..ps-bZIP53_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4304980
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT92960.21593596253641134TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC73580.1709183317924822TruSeq Adapter, Index 7 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27650.028.22753548
TGCCGTC25300.027.39099950
ATGCCGT26500.027.20724549
GTATGCC29000.027.1548747
GCCGTCT21900.026.68920151
CGTATGC28200.026.55998646
CTCGTAT30650.023.98015844
TCGTATG32250.022.03076245
TATCTCG35900.021.64321541
TCTCGTA33950.021.3399743
TCGTATC48300.021.23164738
CGTATCT40650.021.18057639
TATCGCG8200.020.91440238
CCGTCTT30050.020.61541252
CGTATCG11550.019.69674936
ATCTCGT40050.019.48792542
ATTCGTA61500.019.40628836
GTATCTC42650.018.8743540
TTCGTAT59050.018.78895237
CTGCGTG7100.018.23923356
Produced by FastQC (version 0.11.5)