FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP63..ps-bZIP44_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP63..ps-bZIP44_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5830881
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT83060.14244845675979323TruSeq Adapter, Index 13 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC68230.11701490735276539TruSeq Adapter, Index 13 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27050.025.74833748
GTATGCC29300.024.0099847
ATGCCGT27550.023.62950949
CGTATGC30100.022.90673346
TGCCGTC28200.021.59551450
GCCGTCT25200.020.97199451
TCGTATG31850.020.54922945
CTCGTAT30500.020.54076444
CATTCAG58550.018.29186230
CCGTCTT32200.017.9345952
AAGGGGG10300.017.3299170
TCTCGTA36450.016.51559643
CTGCTTG33150.015.09787759
CACATTC72800.014.75945728
ACATTCA77450.014.05407829
ATTCAGA77550.014.03595431
CAGAAAT75350.014.02771834
CTCCAGT76200.013.96310421
CAGTCAC76250.013.86214524
TTCAGAA78450.013.83031732
Produced by FastQC (version 0.11.5)