FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP63..ps-bZIP44_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP63..ps-bZIP44_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7386441
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT117680.15931894670247823TruSeq Adapter, Index 13 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC98930.13393459718963435TruSeq Adapter, Index 13 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG39650.026.12839748
ATGCCGT40350.024.89445149
GTATGCC43000.024.74396947
CGTATGC41900.024.2241346
TGCCGTC40450.023.3619750
GCCGTCT37100.023.3016851
TCGTATG44350.022.4124345
CTCGTAT42500.021.24687244
CCGTCTT47000.019.1381352
CATTCAG83000.018.25887533
TCTCGTA50700.017.9485643
CTGCTTG43900.015.62628659
CACATTC98500.015.527781531
GCTTGAA57150.015.49417861
CAGAAAT101600.014.53727337
CAGTCAC105150.014.51247327
ATTCAGA108800.014.34730134
TTCAGAA108250.014.32319935
AATCTCG69550.014.29175141
CTCCAGT107850.014.01934824
Produced by FastQC (version 0.11.5)