FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP63..ps-bZIP2_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP63..ps-bZIP2_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4245999
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT118030.2779793400799199TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC100050.23563359294243827TruSeq Adapter, Index 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31050.029.98364848
CGTATGC31650.029.52582446
GTATGCC31700.029.47925247
TGCCGTC31400.028.75772550
ATGCCGT31500.028.55531949
GCCGTCT30200.028.27791451
TCGTATG35200.026.0509245
CTCGTAT34800.025.64634144
CCGTCTT33850.025.53893552
CGCTCAT39450.025.19624334
CCGCTCA40800.024.44832633
CTGCTTG33750.023.85165659
TATCTCG37350.023.61426441
ACCGCTC43200.022.84703332
TCTCGTA38050.022.62793543
CACCGCT45050.021.8311231
TCACCGC47050.020.9775130
ATCTCGT42250.020.79272842
GCTCATT47500.020.70509335
CGTCTTC42200.020.15386453
Produced by FastQC (version 0.11.5)