FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP63..ps-bZIP2_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP63..ps-bZIP2_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5026777
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT160130.318554015823658TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC131170.26094254827695756TruSeq Adapter, Index 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41000.031.49961548
ATGCCGT42200.030.18920549
GTATGCC43500.030.17204547
CGTATGC43800.030.12520446
GCCGTCT40600.029.91342751
TGCCGTC42300.029.70412850
TCGTATG47750.027.77976645
CTCGTAT45850.027.63325144
CCGTCTT45900.026.99313552
CGCTCAT53150.026.01097134
TATCTCG49950.025.15484641
TCTCGTA50800.024.66505243
CCGCTCA56800.024.64758733
ACCGCTC57800.023.73673232
CTGCTTG46200.023.56031659
CACCGCT61400.022.4590131
CGTCTTC54700.022.33062253
ATCTCGT56100.022.21006842
GCTCATT64250.021.89856535
TCACCGC64550.021.30880230
Produced by FastQC (version 0.11.5)