FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP63..ps-bZIP1_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP63..ps-bZIP1_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6938339
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG42950.027.6248839
TCGGGGG36250.024.71700340
CGGGGGT19850.024.68490241
AATCGGG50600.024.00174738
TCCGGAG77850.022.20916431
ACTCCGG77400.021.97653429
CACTCCG76850.021.95164528
GAATCGG58850.021.29121437
CTCCGGA80500.021.26066630
AGAATCG67900.019.43280239
CCGGAGA91000.018.65366732
TCACTCC93650.018.23794627
CGGAGAA89300.018.22490933
CGGGGGG26300.017.8325344
GTCACTC97150.017.65294526
CAGTCAC101150.016.78184724
AGTCACT106100.016.36176525
TCCAGTC105750.016.28353122
TCGGGGT16400.015.7925340
CTCCAGT108700.015.68062321