FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP63..ps-bZIP11_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP63..ps-bZIP11_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7455157
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18200.018.65370848
CGTATGC21650.016.65114643
ATGCCGT21000.016.33321246
GCCGTCT20150.015.63263748
GTATGCC22550.015.36573947
TGCCGTC22700.014.33909947
TCGTATG26350.013.94676542
CTCGTAT26200.012.55715841
CCGTCTT30400.010.82228752
TCACGAG38550.010.16853627
CACGAGA38850.09.99992628
GTCACGA40400.09.52963226
TCTCGTA33500.09.29843743
CTGCTTG35350.08.81181556
AGTCACG44150.08.72020628
CGAGATT48150.08.35923230
CGTCTTC45050.07.846778450
CTCCAGT50550.07.54692721
ACGAGAT53500.07.45788829
ACTCCAG52050.07.39667923
Produced by FastQC (version 0.11.5)