FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP25..ps-bZIP53_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP25..ps-bZIP53_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3885171
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT87220.22449462327398204TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC55480.14279937742766022TruSeq Adapter, Index 7 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23100.035.45408648
GCCGTCT19550.035.26808551
TGCCGTC22700.034.38281650
CGTATGC24000.033.54123346
ATGCCGT24200.033.26403449
GTATGCC24850.032.9573247
CCGTCTT23850.029.93672452
CTCGTAT26750.027.34544244
TCGTATG27950.026.92273145
CGTATCT32400.026.14163839
TCGTATC37550.025.91178138
TATCTCG31300.024.48850341
GTATCTC33750.023.6441440
TCTCGTA30600.023.56178943
CTGCTTG23750.022.98917859
ATTCGTA46750.022.45989236
ATCTCGT34400.021.97646142
TTCGTAT47250.021.48120537
CACGAAT50750.021.24137931
AATTCGT52300.020.61185535
Produced by FastQC (version 0.11.5)