FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP25..ps-bZIP53_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP25..ps-bZIP53_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5772483
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT129050.22356064106208712TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC105630.18298884552799896TruSeq Adapter, Index 7 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36650.028.07597548
CGTATGC37800.027.59217546
GCCGTCT30100.027.44144651
TGCCGTC35050.027.1607950
GTATGCC39300.027.07338347
ATGCCGT36100.026.37079249
CTCGTAT40250.023.73877141
TCGTATC61600.022.55647935
TATCTCG48500.022.29863241
TCGTATG44550.022.15454945
CCGTCTT40350.021.77166652
CGTATCT54300.021.01257539
TCTCGTA45250.020.80631443
TATCGCG10550.020.5684138
ATCTCGT50350.020.29760742
ATTCGTA78450.019.89772233
GTATCTC56550.019.49572840
TTCGTAT77150.019.0988734
CGTCTTC44500.018.48286653
CGTATCG14950.018.26059336
Produced by FastQC (version 0.11.5)