FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP25..ps-bZIP44_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP25..ps-bZIP44_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3455426
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT68130.19716816392537417TruSeq Adapter, Index 13 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC57180.16547887293780855TruSeq Adapter, Index 13 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT19300.029.37792246
TATGCCG19950.028.94705845
TGCCGTC20000.027.999747
GCCGTCT17350.027.63659148
CGTATGC21450.026.92278743
GTATGCC22250.026.89858844
TCGTATG24200.023.71875442
CTCGTAT21500.022.79045341
CCGTCTT22000.021.47704149
CTGCTTG21450.020.72238756
AAGGGGG6550.020.30512470
TCTCGTA25050.019.70038640
ATCTCGT27750.018.41421739
AATCTCG29350.018.1258738
CATTCAG49650.017.69366630
CTGCGTG4600.017.49981356
CGTCTTC28350.016.66648750
AATCGCG5800.016.29292941
GCTTGAA30700.015.96073958
CTTCTGC28500.015.71912853
Produced by FastQC (version 0.11.5)