FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP25..ps-bZIP44_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP25..ps-bZIP44_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4099678
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT78340.1910881781447226TruSeq Adapter, Index 13 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC58760.14332832968833162TruSeq Adapter, Index 13 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23100.030.30262248
GTATGCC23950.029.51943847
TGCCGTC20950.029.06882350
CGTATGC24100.028.90002846
ATGCCGT23550.028.23741349
GCCGTCT20000.027.82462551
CTCGTAT26650.025.08409344
TCGTATG28100.024.91069845
CCGTCTT25400.022.4603352
TCTCGTA31250.020.9437243
AATCTCG35850.019.9160541
ATCTCGT35000.019.49973942
CATTCAG53950.018.35934633
CTGCTTG24650.017.74824159
CGTCTTC31500.016.33311553
TGCCGGC4200.015.8331250
GCTTGAA36100.015.7061661
CAGTCAC63350.015.69039727
CACATTC64450.015.36829631
CAGAAAT63850.015.34826837
Produced by FastQC (version 0.11.5)