FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP25..ps-bZIP2_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP25..ps-bZIP2_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3114910
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT100060.32122918479185597TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC88820.2851446751270502TruSeq Adapter, Index 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26900.029.92540448
ATGCCGT26950.029.74001549
TGCCGTC26550.029.52894650
GCCGTCT25750.029.35898251
CGTATGC27700.029.18748546
GTATGCC28700.028.5363547
CCGTCTT29550.025.8204352
TCGTATG30850.025.4130845
CTCGTAT30600.025.04881344
TATCTCG31500.023.8886941
CTGCTTG29150.023.65331859
TCTCGTA32700.023.22610743
CGCTCAT37800.022.7775934
ATCTCGT33750.022.1924142
CGTCTTC34700.021.88742653
CCGCTCA39250.021.84695233
ACCGCTC41000.020.99982632
CTTCTGC34450.020.82711456
CACCGCT42450.020.36496731
GCTCATT44700.019.88797835
Produced by FastQC (version 0.11.5)