FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP25..ps-bZIP2_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP25..ps-bZIP2_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3700921
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT110350.2981690233323002TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC95940.25923276935660067TruSeq Adapter, Index 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC29200.030.44482647
TATGCCG29500.030.13521848
GCCGTCT26550.029.92429451
ATGCCGT29550.029.61045649
CGTATGC30150.029.60161846
TGCCGTC29000.029.08584250
TCGTATG34550.026.23700745
CTCGTAT32600.026.08862944
CCGTCTT32000.025.48405552
CGCTCAT38000.024.59179734
TATCTCG35500.023.76026541
TCTCGTA36000.023.4302643
CCGCTCA40800.022.98990633
ATCTCGT38000.022.47340242
CTGCTTG31550.022.2976659
ACCGCTC41150.021.9438332
GCTCATT44800.021.17160835
CGTCTTC38400.021.05442453
CTTCTGC36700.020.88529856
CACCGCT44500.020.44918331
Produced by FastQC (version 0.11.5)