FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP25..ps-bZIP1_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP25..ps-bZIP1_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3588305
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT37560.1046733764270317TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG36280.1011062326084321TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG26150.027.83921639
TCGGGGG22050.025.07920840
CGGGGGT11850.024.21926141
AATCGGG32150.024.16781438
GAATCGG35950.022.00264537
TCCGGAG50300.021.4312834
ACTCCGG50100.021.16753232
CTCCGGA51300.021.01351533
CCGGAGA50150.020.65789835
CGGAGAA49450.020.38410236
AGAATCG41000.020.14621736
CACTCCG53100.019.90571631
CGGGGGG15800.019.27203244
GGAGAAT54050.017.61321337
TCACTCC61850.017.37256830
GTCACTC62750.017.01184729
TCGGGGT9400.016.75521540
GAGAATC52800.016.43929335
TCGGGTG3701.8189894E-1216.08098243
CAGTCAC66700.016.00439827
Produced by FastQC (version 0.11.5)